Identification of the Bacteria that causes Childhood Tonsillitis
Abstract
Introduction: Tonsillitis is the most common infectious condition after viral nose and throat infections. Millions of new infections every year are recorded globally. Young people under the age of 15 are disproportionately affected by acute tonsillitis. This study aimed to isolate the bacteria causing tonsilitis in children.
Methods: One hundred thirty throat swabs were taken from a variety of individuals who were clinically determined to have tonsillitis with purulent discharges at the ENT clinic at Kuthospital. Isolates were tested for their gram stain response and biochemical features after being promptly transported to the laboratory and streaked directly on blood agar, which was incubated aerobically for 24 hours at 37°C. The following antibiotics were tested on all isolates using the disc diffusion technique: penicillin (10 units), gentamicin (10 mcg), vancomycin (30 mcg), erythromycin (15 mcg), ciprofloxacin (10 mcg), cephalothin (30 mcg), and chloramphenicol (30 mcg). Isolates were classified as sensitive or resistant based on the widths of zones of inhibition.
Results: Thirty-four strains of Streptococci, 14 strains of Staphylococcus epidermidis, 32 strains of Staphylococcus aureus, 15 strains of another Staphylococcus spp., and 9 strains of Haemophilus parainfluenzae were detected. The current results showed that most isolates were sensitive to ciprofloxacin, chloramphenicol, and vancomycin, while most isolates showed resistance to penicillin and gentamycin.
Conclusion: Streptococci and S. aureus showed to be the most common bacterial causes of tonsilitis in children who were sensitive to ciprofloxacin, chloramphenicol, and vancomycin.
How to cite this article:
Sakr FM, Fadhil AA, Rubaye AWAL, Jawad MA, Khaled DW, Alwan NH, Al-Jassani MJ. Identification of the Bacteria that causes Childhood Tonsillitis. J Commun Dis. 2023;55(1):102-105.
DOI: https://doi.org/10.24321/0019.5138.202316
References
Standring S. Pharynx. Chapter 35. In: Gray’s anatomy: the anatomical basis of clinical practice. 39th ed. Philadelphia: Elsevier; 2005. p. 619-31.
Wald ER, Green MD, Schwartz B, Barbadora K. A streptococcal score card revisited. Pediatr Emerg Care. 1998;14(2):109-11. [PubMed] [Google Scholar]
National Center for Health Statistics. National ambulatory medical care survey. US Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Center for Health Statistics; 2000. [Google Scholar]
Komaroff AL, Pass TM, Aronson MD, Ervin CT, Cretin S, Winickoff RN, Branch Jr WT. The prediction of streptococcal pharyngitis in adults. J Gen Intern Med. 1986;1(1):1-7. [PubMed] [Google Scholar]
Kaplan EL, Top Jr FH, Dudding BA, Wannamaker LW. Diagnosis of streptococcal pharyngitis: differentiation of active infection from the carrier state in the symptomatic child. J Infect Dis. 1971;123(5):490-501. [PubMed] [Google Scholar]
Schroeder BM. Diagnosis and management of group A streptococcal pharyngitis. Am Fam Physician. 2003;67(4):880-4. [PubMed] [Google Scholar]
Mücke W, Huber HC, Ritter U. Mitteilung über die Keimbesiedelung der Gaumenmandeln bei gesunden Kindern im Einschulalter. Zentralbl Hyg Umweltmed. 1994;196(1):70-4. German. [PubMed] [Google Scholar]
Schwaab M, Gurr A, Hansen S, Minovi AM, Thomas JP, Sudhoff H, Dazert S. Human beta-Defensins in different states of diseases of the tonsilla palatina. Eur Arch Otorhinolaryngol. 2010;267(5):821-30. [PubMed] [Google Scholar]
Jensen A, Fagö-Olsen H, Sørensen CH, Kilian M. Molecular mapping to species level of the tonsillar crypt microbiota associated with health and recurrent tonsillitis. PLoS One. 2013;8(3):e56418. [PubMed] [Google Scholar]
Brandtzaeg P. Immune functions of human nasal mucosa and tonsils in health and disease. In: Immunology of the lung and upper respiratory tract. McGraw-Hill; 1984. p. 28-95. [Google Scholar]
Windfuhr JP, Toepfner N, Steffen G, Waldfahrer F, Berner R. Clinical practice guideline: tonsillitis I. Diagnostics and nonsurgical management. Eur Arch Otorhinolaryngol. 2016;273(4):973-87. [PubMed] [Google Scholar]
Barrow GI, Feltham RK. Cowan and steel’s manual for the identification of medical bacteria. 3rd ed. Cambridge University; 2012.
National Committee for Clinical Laboratory Standards. Approval standard M7-A4: methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 4th ed. Villanova, Pennsylvannia: National Committee for Clinical Laboratory Standards; 1997.
Sun J, Keh-Gong W, Hwang B. Evaluation of the etiologic agents for acute suppurative tonsillitis in children. Zhonghua Yi Xue Za Zhi (Taipei). 2002;65(5):212-7. [PubMed] [Google Scholar]
Strohl WA, Rouse H, Fisher BD. Lippincott’s illustrated reviews: microbiology. Harvey RA, editor. USA: Lippincott Williams and Wilkins; 2015. p. 137-55, 191-3.
Pyatkin KD, Krivoshein YS. Microbiology with virology and immunology. Revised ed. Union of Soviet Socialist Republics. Moscow: MIR Publishers; 2005. p. 281-99, 365-68.
Walsh BT, Bookheim WW, Johnson RC, Tompkis RK. Recognition of streptococcal pharyngitis in adults. Arch Intern Med. 1975;135(11):1493-7. [PubMed] [Google Scholar]
Pratt L. Infections in the lymphoid tissue. In: English GM, editor. Otolaryngology. Vol. 3. 1986.
Philadelphia; JF Lippincott;.Pichichero Michael ME, Disney FA, Aronovitz GH, et al. Randomized, Singleblind Evaluation of Cefadroxil and Phenoxymethyl; Antimicrob Agents chemother; 2007; 31: 903-6.
Pichichero ME. Explanations and therapies for penicillin failure in streptococcal pharyngitis. Clin Pediatr (Phila). 1992;31(11):642-9. [PubMed] [Google Scholar]
Kelley R, Langley G, Bates L. Erythromycin. Still a good choice for strep throat. Clin Pediatr (Phila). 1993;32(12):744-5. [PubMed] [Google Scholar]
Seppala H, Nissinen A, Jarvinen H, Huovinen S, Henriksson T, Herva E, Holm SE, Jahkola M, Katila ML, Klaukka T, Kontiainen S, Liimatainen O, Oinonen S, Passi-Metsomaa L, Huovinen P. Resistance to erythromycin in group A streptococci. N Engl J Med. 1992 Jan 30;326(5):292-7. [PubMed] [Google Scholar]
Hueston WJ, Dickerson L. Antibiotic resistance and the need for the rational use of antibiotics. J Med Liban. 2001;49(5):246-56. [PubMed] [Google Scholar]
Copyright (c) 2023 Authors
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.