Monitoring of Insecticide Resistance and Exploring the Presence of Virus in Field Populations of Culex gelidus at Thiruvarur District of Tamil Nadu, India

  • Rajalakshmi Anbalagan Department of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, India.
  • Arpita Shukla Department of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, India.
  • Vimala Subramanian Department of Epidemiology and Public Health, Central University of Tamil Nadu, Thriuvarur, Tamil Nadu, India.
  • PK Srivastava Former Joint Director, National Vector Borne Disease Control Programme, Delhi, India.
  • Jayalakshmi Krishnan Department of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, India.
Keywords: Cx. gelidus, DDT, Deltamethrin, Malathion, Thiruvarur, WHO Susceptibility Test


Introduction: The introduction of potent synthetic insecticides into public health programmes has since beginning posed the challenge of development of resistance among the insect vectors against the insecticides. Culex (Cx.) gelidus is one of the vectors of JE which is abundantly found in southern India. Its breeding habitats are similar to the vishnui subgroup of Culex mosquitoes, the major vectors for transmission of JE. The present study was aimed to assess the susceptibility status of adult Culex (Cx.) gelidus, to insecticides, namely DDT (Dichlorodiphenyltrichloroethane), Deltamethrin, and Malathion.

Method: The field-collected mosquito larvae from ten villages of Thiruvarur district from December 2018 to May 2019 were reared in the laboratory until F1 generation and the emerged adults identified as Cx. gelidus were exposed to insecticide-impregnated papers supplied through World Health Organization (WHO). The adult susceptibility tests were carried out as per the protocol of WHO. Further, an attempt was made to check the presence of JE virus in Cx. gelidus and the virus detection was done by RT-PCR.

Results: The results indicated that the adult Cx. gelidus populations were susceptible to DDT, whereas they were resistant to Malathion and Deltamethrin. The possible reason of DDT susceptibility may be that DDT has not been used in Tamil Nadu since the year 1980 (about 40 years).

Conclusion: JE virus was not detected in the tested mosquitoes. The study suggests that insecticide resistance monitoring from time to time is required to facilitate vector control programmes in focusing on appropriate vector control measures.

How to cite this article:
Anbalagan R, Shukla A, Subramanian V, Srivastava PK, Krishnan J. Monitoring of Insecticide Resistance and Exploring the Presence of Virus in Field Populations of Culex gelidus at Thiruvarur District of Tamil Nadu, India. J Commun Dis. 2021;53(4):76-83.



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