Detection of Dengue Virus in Human Blood Samples from Selected Localities of Delhi and NCR
Abstract
A survey for detection of dengue virus in human blood was conducted. The blood filter paper blots of suspected cases of dengue were collected from different localities of Delhi and NCR. These blood spots were tested using rapid diagnostic kits. In all 87 suspected cases were tested for the presence of dengue virus by repeating them for all confirmation. Out of 87 samples 20 were found dengue positive, 56 samples had past infection and 5 cases were having current fever. The positive samples were again confirmed by PCR and about 8 cases were found positive out of 20 cases. The positivity was found very high from October to November 2016. The Sero conversion rate shows that about 35% of resident are getting new infection each year which is indicator of increasing local transmission. RT PCR can detect low viramia which helps in isolating patient and timely control activities. PCR technique along with conventional kits should be carried out to access cases more accurately, so that control can be targeted to such areas.
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