Role of Pro-inflammatory IL-8 and Anti-inflammatory IL-10 Cytokines in Dengue Severity
Abstract
Introduction: During dengue infection, cytokine levels may increase as various cytokines are released from infected inflammatory cells. This study was conducted to measure the levels of cytokines IL-8 and IL-10 in dengue patients and correlate them with dengue severity.
Material & Methods: A prospective study was conducted on febrile patients suspected of dengue fever, seeking medical care in our institute. 107 cases confirmed to have dengue fever (by NS1/ IgM ELISA) and 100 healthy individuals with age and sex matched, were included in the study. The clinical features of all patients were recorded, and cytokine levels of IL-8 and IL-10 were estimated by ELISA in the dengue patients and healthy controls.
Results: Out of 400 febrile patients suspected of having dengue fever, 107 (26.75%) cases were confirmed cases, of which 56 (52.3%), 20 (18.7%), and 31 (29%) were positive for only NS1 antigen, only IgM antibody, and both NS1 and IgM, respectively. Depending on the severity of the disease, 9 (8.5%) cases were classified as severe dengue cases while 98 (91.5%) as non-severe dengue fever. Mean levels (pg/ml) for IL-8 were 281.6 ± 76.6, 150.41 ± 55.9 and 75.4 ± 49.2 in severe dengue, dengue fever, and healthy controls respectively while for IL-10, the values were 219.4 ± 150.5, 38.9 ± 67.2, and 6.6 ± 0.65 among severe dengue cases, dengue cases, and healthy controls, respectively.
Conclusion: Mean level of cytokines IL-8 and IL-10 were significantly raised in severe dengue patients as compared to non-severe dengue patients and healthy controls, suggesting their role in causing severe disease and as a potential predictor for disease severity and fatal outcome.
How to cite this article:
Sharma M, Chattopadhya D, Chakravarti A, Gill PS, Yumnam H. Role of Pro-inflammatory IL-8 and Anti-inflammatory IL-10 Cytokines in Dengue Severity. J Commun Dis. 2021; 53(2): 69-75.
DOI: https://doi.org/10.24321/0019.5138.202128
References
Mayer SV, Tesh RB, Vasilakis N. The emergence of arthropod-borne viral diseases: A global prospective
on dengue, chikungunya and zika fevers. Acta Trop. 2017;166:155-63. [PubMed] [Google Scholar]
Martina BEE, Koraka P, Osterhaus ADME. Dengue virus pathogenesis: an integrated view. Clin Microbiol Rev.
Oct;22(4):564-81. [PubMed] [Google Scholar]3. Yung CF, Lee KS, Thein TL, Tan LK, Gan VC, Wong JGX, Lye
DC, Ng LC, Leo YS. Dengue serotype-specific differences in clinical manifestation, laboratory parameters and risk
of severe disease in adults, Singapore. Am J Trop Med Hyg. 2015;92(5):999-1005. [PubMed] [Google Scholar]
Rothman AL. Immunity to dengue virus: A tale of original antigenic sin and tropical cytokine storms. Nat
Rev Immunol. 2011;11(8):532-43. [PubMed] [Google Scholar]
Screaton G, Mongkolsapaya J, Yacoub S, Roberts C. New insights into the immunopathology and
control of dengue virus infection. Nat Rev Immunol. 2015;15(12):745-59. [PubMed] [Google Scholar]
Waggoner JJ, Balmaseda A, Gresh L, Sahoo MK, Montoya M, Wang C, Abeynayake J, Kuan G, Pinsky
BA, Harris E. Homotypic Dengue Virus Reinfections in Nicaraguan Children. J Infect Dis. 2016;214(7):986-93.
[PubMed] [Google Scholar]
Beard JA, Bearden A, Striker R. Vitamin D and the antiviral State. J Clin Virol. 2011;50(3):194-200.
[PubMed] [Google Scholar]
Abhishek KS, Chakravarti A, Baveja CP, Kumar N, Siddiqui O, Kumar S. Association of interleukins -2, -4, and
-10 with dengue severity. Indian J Pathol Microbiol. 2017;60(1):66-9. [PubMed] [Google Scholar]
Pinto LM, Oliveira SA, Braga EL, Nogueira RM, Kubelka CF. Increased proinflammatory cytokines (TNF-α and
IL-6) and anti-inflammatory compounds (sTNFRp55 and STNFRp75) in Brazilian patients during exanthematic
dengue fever. Mem Inst Oswaldo Cruz. 1999;94(3):387-94. [PubMed] [Google Scholar]
World Health Organization [Internet]. Dengue: Guidelines for Diagnosis, Treatment, Prevention and
Control. Geneva, Switzerland: WHO; 2009. Available from: https://apps.who.int/iris/handle/10665/44188
[Google Scholar]
Guabiraba R, Ryffel B. Dengue virus infection: current concepts in immune mechanisms and lessons from
murine models. Immunol. 2014;141(2):143-56. [PubMed] [Google Scholar]
Savargaonkar D, Sinha S, Srivastava B, Nagpal BN, Sinha A, Shamim A, Das R, Pande V, Anvikar AR, Valecha N. An epidemiological study of dengue and its coinfections in Delhi. Int J Infect Dis. 2018;74:41-6. [PubMed] [Google
Scholar]
Clyde K, Kyle JL, Harris E. Recent advances in Deciphering Viral and Host determinants of Dengue virus Replication and Pathogenesis. J Virol. 2006;80(23):11418-31. [PubMed] [Google Scholar]
Gupta E, Dar L, Kapoor G, Broor S. The changing epidemiology of dengue in Delhi, India. Virol J.
;3:92. [PubMed] [Google Scholar]
Agarwal R, Kapoor S, Nagar R, Misra A, Tandon R, Mathur A, Misra AK, Srivastava KL, Chaturvedi UC. A
clinical study of the patients with dengue hemorrhagic fever during the epidemic of 1996 at Lucknow,
India. Southeast Asian J Trop Med Public Health. 1999;30(4):735-40. [PubMed] [Google Scholar]
Ray G, Kumar V, Kapoor AK, Dutta AK, Batra S. Status of antioxidants and other biochemical abnormalities in
children with dengue fever. J Trop Pediatr. 1999;45(1):4-7. [PubMed] [Google Scholar]
Wali JP, Biswas A, Handa R, Aggarwal P, Wig N, Dwivedi SN. Dengue haemorrhagic fever in adults: A prospective study of 110 cases. Trop Doct. 1999;29(1):27-30. [PubMed] [Google Scholar]
Ralapanawa U, Alawattegama ATM, Gunrathne M, Tennakoon S, Kularatne SAM, Jayalath T. Value of
peripheral blood count for dengue severity prediction. BMC Res Notes. 2018;11(1):400. [PubMed] [Google
Scholar]
Samanta J, Sharma V. Dengue and its effects on liver. World J Clin Cases. 2015;3(2):125-31. [PubMed] [Google
Scholar]
Mahmuduzzaman M, Chowdhury AS, Ghosh DK, Kabir IM, Rahman MA, Ali MS. Serum transaminase
level changes in dengue fever and its correlation with disease severity. Mymensingh Med J. 2011;20(3):349-
[PubMed] [Google Scholar]
Pancharoen C, Rungsarannont A, Thisyakorn U. Hepatic dysfunction in dengue patients with various severity. J Med Assoc Thai. 2002;85:S298-301. [PubMed] [Google Scholar]
Shivkar RR, Padwal MK, Vaidya A. Correlation of Liver Transaminases with Platelet count in Dengue patients
from Tertiary Care Hospital in Western India. Int J Cur Res Rev. 2018;10(5):15-22.
Romagnani S. Th1/Th2 cells. Inflamm Bowel Dis. 1999;5(4):285-94. [PubMed] [Google Scholar]
Maneekan P, Leaungwutiwong P, Misse D, Luplertlop N. T helper (Th) 1 and Th2 cytokine expression profile
in dengue and malaria infection using magnetic beadbased bio-plex assay. Southeast Asian J Trop Med Public
Health. 2013;44(1):31-6. [PubMed] [Google Scholar]
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