Insecticides Susceptibility Status of Malaria Vectors in a High Malaria Endemic Tribal District Gadchiroli (Maharashtra) of India

  • RK Singh ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India.
  • Gaurav Kumar ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India.
  • Kunal S Modak DMO, Gadchiroli, Maharashtra, India.
  • Rajesh R Karlekar NVBDCP Consultant, DMO Office, Gadchiroli, Maharashtra, India.
  • RC Dhiman ICMR-National Institute of Malaria Research, Sector-8, Dwarka, New Delhi, India.
Keywords: Malaria Vector, Insecticide Susceptibility, Gadchiroli District of Maharashtra, India


Background and Objective: The current study was undertaken to determine insecticide susceptibility of malaria vectors in various villages of high malaria endemic PHCs of Gadchiroli district of Maharashtra.

Methods: Adult malaria vectors were collected from the human dwellings/ cattle sheds of 156 villages of 18 malaria endemic PHCs. Susceptibility tests were carried out for different insecticides against An. culicifacies and An. fluviatilis mosquitoes as per the World Health Organization (WHO) procedure. Cone bioassays were also done to assess the quality and efficacy of indoor residual spray.

Results: An. fluviatilis could be collected from 23 villages only and all the populations were fully susceptible to synthetic pyrethroid (deltamethrin) while being tolerant to organophosphorous (malathion). Susceptibility of An. culicifacies from 156 villages indicated that only 3 populations of An. culicifacies were resistant to deltamethrin while 57 populations were fully susceptible and other 96 populations were tolerant to deltamethrin. Resistance was recorded in 25 populations of An. culicifacies against malathion and 30 populations were tolerant to malathion insecticide. Remaining populations of An. fluviatilis and
An. culicifacies were highly resistant to organochlorine. Results of cone bioassay revealed the mortality ranged from 32.5-51.1% on cemented and 27.5-43.3% on the mud wall sprayed with lambda cyhalothrin.

Conclusion: The current study indicates that resistance has developed to synthetic pyrethroids in the major malaria vector An. culicifacies. Therefore, there is an urgent need for the evaluation of new insecticide
molecules for better control of malaria vectors.

How to cite this article:
Singh RK, Kumar G, Modak KS, Karlekar RR, Dhiman RC. Insecticides Susceptibility Status of Malaria Vectors in a High Malaria Endemic Tribal District Gadchiroli (Maharashtra) of India. J Commun Dis. 2021;53(3):33-50.



World Health Organization [Internet]. World Malaria Report 2018; [cited 2020 Nov 20]. Available


National Vector Borne Disease Control Programme [Internet]. Malaria situation in India, Delhi; [cited

Nov 20]. Available from:

Kumar A, Valecha N, Jain T, Dash AP. Burden of Malaria in India: retrospective and prospective view. Am J Trop

Med Hyg. 2007;77(6 Suppl):69-78. [PubMed]

Dhingra N, Joshi RD, Dhillon GP, Lal S. Enhanced Malaria Control Project for World Bank support under National Malaria Eradication Programme (NMEP). J Commun Dis. 1997;29(3):201-8. [PubMed] [Google Scholar]

Office of District Malaria Office, Gadchiroli, Maharashtra state.


Sharma VP. Continuing challenge of malaria in India. Curr Sci. 2012;102(5):678-82. [Google Scholar]

Singh RK, Kumar G, Mittal PK. Insecticide susceptibility status of malaria vectors in India: A review. Int J Mosq

Res. 2014;1(1):5-9. [Google Scholar]

Sharma VP, Dev V. Biology & control of Anopheles culicifacies Giles 1901. Ind J Med Res. 2015;141(5):525-

[PubMed] [Google Scholar]

Sharma VP. Fighting malaria in India. Curr Sci. 1998;75:1127-40. [Google Scholar]

Rahman J, Roy ML, Singh K. Development of increased tolerance to DDT in Anopheles culicifacies Giles, in the

Panch Mahal district of Bombay state, India. Indian J Malariol. 1959;13(2/3):125-30. [Google Scholar]

Rajagopal R. Malathion resistance in Anopheles culicifacies in Gujarat. Indian J Med Res. 1977;66:27-

[PubMed] [Google Scholar]

Dhingra N, Joshi RD, Dhillan GP, Lal S. Enhanced Malaria Control Project by World Bank support under National Malaria Eradication Program (NMEP). J Commun Dis. 1997; 29:201-208.

Government of Maharashtra Public Health Department, Directorate of Health Services, [cited

Nov 1]. Available from:


Singh OP, Raghavendra K, Nanda N, Mittal PK, Subbarao SK. Pyrethroid resistance in An. culicifacies in Surat

district, Gujarat, west India. Curr Sci. 2002;82:547-50. [Google Scholar]

Office of the Registrar General & Census Commissioner, India [Internet]. Census of India. 2011. Available

from: (accessed on 26.11.2021)

World Health Organization [Internet]. Instructions for determining the susceptibility or resistance of

adult mosquito to organo-chlorine organophosphate and carbonate insecticides – Diagnostic test. WHO/


World Health Organization. Manual on practical entomology in malaria part II. Methods and techniques.

Geneva: World Health Organization; 1975. 191 p.

Wattal BL, Kalra NL. Regionwise pictorial keys to the female Indian Anopheles. Bull Nat Soc Mal Other Mosqu

Borne Dis. 1961;9:85-138. [Google Scholar]

Das BP, Rajagopal R, Akiyama J. Pictorial key to the species of Indian Anopheline mosquitoes. J Pure Appl

Zool. 1990;2:131-62. [Google Scholar]

Abbott WS. A method of computing the effectiveness of an insecticide. J Econ Entomol. 1925;18:265-7.

WHO. Instructions for the bioassay of insecticidal deposits on wall surfaces. VBC/81.5 (WHO/VBC/81.812).

Geneva: World Health Organization; 1981.

Singh RK, Dhiman RC, Mittal PK, Das MK. Susceptibility of Malaria Vectors to insecticides in Gumla

district, Jharkhand state, India. J Vector Borne Dis. 2010;47(2):116-8. [PubMed] [Google Scholar]

Singh RK, Dhiman RC, Das MK. Situation analysis of malaria in Godda district of Jharkhand during malaria

epidemic. J Commun Dis. 2011;43(2):135-42. [PubMed] [Google Scholar]

Singh RK, Mittal PK, Gourshettiwar MP, Pande SJ, Dhiman RC. Susceptibility of malaria vectors to insecticides in Gadchiroli district (Maharashtra), India. J Vector Borne Dis. 2012;49(1):42-4. [PubMed] [Google Scholar]

Dhiman RC, Shahi B, Sharma SN, Nanda N, Khargiwarkar VN, Subbarao SK. Persistence of malaria transmission in a tribal area in Maharashtra, India. Curr Sci. 2005;88(3):475-8. [Google Scholar]

Rao TR, Bhatia SC. A note on the degree of susceptibility of Anopheles culicifacies to DDT in some parts of

Bombay state. Indian J Malariol. 1957;11(3):261-9. [PubMed] [Google Scholar]

Deobhankar RB, Palkar ND. Magnitude of DDT resistance in Anopheles culicifacies in Maharashtra

state. J Commun Dis. 1990;22:77. [PubMed] [Google Scholar]

Krishnamurthy BS, Singh NN. DDT resistance in Anopheles culicifacies Giles, 1901 and Anopheles

annularis Van der Wulp 1884 in a village of Meerut district, U.P. Indian J Malariol. 1962;16:375-7. [Google


Vittal M, Deshpande LB. Development of Malathion resistance in a DDT, HCH resistant Anopheles culicifacies

population in Thane district (Maharashtra). J Commun Dis. 1983;15:144-5. [PubMed] [Google Scholar]

Sharma MID, Samnotra KG. A note on gamma BHC and dieldren resistance in Anopheles culicifacies Giles

in adjoining areas of Gujarat and Maharashtra states. Bull Natl Soc India Malar Mosq Dis. 1962;10:151-7.31. Singh RK, Dhiman RC, Kumar G, Sinha ATS, Dua VK. Susceptibility status of malaria vectors to insecticides

in Koderma, Jharkhand. J Commun Dis. 2011;43:273-6. [PubMed] [Google Scholar]

Sharma SK, Upadhyay AK, Haque MA, Singh OP, Adak T, Subbarao SK. Insecticide susceptibility status of

malaria vectors in some hyper-endemic tribal districts of Orissa. Curr Sci. 2004;87(12):1722-6.

Sahu SS, Gunasekaran K, Vijayakumar T, Jambulingam P. Triple insecticide resistance in Anopheles culicifacies: a practical impediment for malaria control in Odisha State, India. Indian J Med Res. 2015 Dec;142(Suppl 1):S59-63. [PubMed] [Google Scholar]

Raghavendra K, Vasantha K, Subbarao SK, Pillai MK, Sharma VP. Resistance in Anopheles culicifacies sibling

species B and C to Malathion in Andhra Pradesh and Gujarat states, India. J Am Mosq Control Assoc.

;7(2):255-9. [PubMed] [Google Scholar]

Mishra AK, Chand SK, Barik TK, Dua VK, Raghavendra K. Insecticide resistance status in Anopheles culicifacies

in Madhya Pradesh, central India. J Vector Borne Dis. 2012;49(1):39-41. [PubMed] [Google Scholar]

Bhatt RM, Sharma SN, Barik TK, Raghavendra K. Status of insecticide resistance in malaria vector, Anopheles

culicifacies in Chhattisgarh state, India. J Vector Borne Dis. 2012;49(1):36-8. [PubMed] [Google Scholar]

Chand G, Behera P, Bang A, Singh N. Status of insecticide resistance in An. culicifacies in Gadchiroli (Maharashtra) India. Pathog Glob Health. 2017;111(7):362-6. [PubMed] [Google Scholar]

Singh N, Khare KK. Forest malaria in Madhya Pradesh: changing scenario of disease and its vectors. Indian J

Parasit Dis. 1999;23:105-12.

Sahu SS, Patra KP. A study on insecticides resistance in Anopheles fluviatilis and An. culicifacies to HCH and DDT in the Malkangiri district of Orissa. Indian J Malariol. 1995;32(3):112-8. [PubMed] [Google Scholar]

Koekemoer LL, Spillings BL, Christian RN, Lo TC, Kaiser ML, Norton RA, Oliver SV, Choi KS, Brooke BD, Hunt

RH, Coetzee M. Multiple insecticide resistance in Anopheles gambiae (Diptera: Culicidae) from Pointe

Noire, Republic of the Congo. Vector Borne Zoonotic Dis. 2011;11:1193-200. [PubMed] [Google Scholar]

Dabiré KR, Diabaté A, Djogbenou L, Ouari A, N’Guessan R, Ouedraogo JB, Hougard JM, Chandre F, Baldet T.

Dynamics of multiple insecticide resistance in the malaria vector Anopheles gambiae in a rice growing area

in South-Western Burkina Faso. Malar J. 2008;7(1):188. [PubMed] [Google Scholar]

Djouaka RJ, Atoyebi SM, Tchigossou GM, Riveron JM, Irving H, Akoton R, Kusimo MO, Bakare AA, Wondji

CS. Evidence of a multiple insecticide resistance in the malaria vector Anopheles funestus in South West

Nigeria. Malar J. 2016;15(1):565. [PubMed] [Google Scholar]

Nardini L, Christian RN, Coetzer N, Koekemoer LL. DDT and pyrethroid resistance in Anopheles arabiensis from South Africa. Parasit Vectors. 2013;6(1):229. [PubMed] [Google Scholar]

Toé KH, N’Falé S, Dabiré RK, Ranson H, Jones CM. The recent escalation in strength of pyrethroid resistance in

Anopheles coluzzi in West Africa is linked to increased expression of multiple gene families. BMC Genomics.

;16:146. [PubMed] [Google Scholar]

Gunasekaran K, Sahu SS, Jambulingam P, Das PK. DDT Indoor Residual spray, still an effective tool to

control Anopheles fluviatilis-transmitted Plasmodium falciparum malaria in India. Trop Med Int Health.

;10:160-8. [PubMed] [Google Scholar]

Shukla RP, Sharma SN, Bhat SK. Malaria outbreak in Bhojpur PHC of District Moradabad, Uttar Pradesh,

India. J Commun Dis. 2002;34:118-23. [PubMed] [Google Scholar]

Vittal M, Bhate MR. Bioassay tests on the effectiveness of malathion spraying on Anopheles culicifacies resting on different wall surfaces in Aurangabad town, Maharashtra. Indian J Malariol. 1981;18:124-5.