Cross-sectional Study on Sero-Prevalence of SARS-CoV-2 Infection in Jabalpur, Madhya Pradesh, India

  • Sudarshan Ramaswamy World Heath Organization (WHO) Country Office, Delhi, India.
  • Aditya Athotra National centre for Disease Control, New Delhi
  • PK Kasar Government Medical College, Jabalpur, Madhya Pradesh, India.
  • Rajesh Tiwari Government Medical College, Jabalpur, Madhya Pradesh, India.
  • Shashi Prabha Tomar Government Medical College, Jabalpur, Madhya Pradesh, India.
  • Ratnesh Kuraria Government Medical College, Jabalpur, Madhya Pradesh, India.
  • Riti Jain seth Government Medical College, Jabalpur, Madhya Pradesh, India.
  • Arvind Rai National Centre for Disease Control, Delhi, India.
  • Meera Dhuria National Centre for Disease Control, Delhi, India.
  • Arti Bahl National Centre for Disease Control, Delhi, India.
  • Anil Digambar Patil National Centre for Disease Control, Delhi, India.
  • SK Jain National Centre for Disease Control, Delhi, India.
  • Sujeet K Singh National Centre for Disease Control, Delhi, India.
Keywords: Jabalpur, Seroprevalence, SARS-CoV-2, Community based, Infections


Introduction: In India, laboratory diagnosis of SARS - CoV-2 infection has been mostly based on real-time reverse transcriptase polymerase chain reaction (RT-PCR). Studies have shown that Viral titres peak within the first week of symptoms, but may decline post this time frame, thereby hampering RT-PCR based diagnostic strategies. These reasons have prompted the call for adoption of antibody testing as a potential source of data to address the gap in data and inform public health and governance policies oriented towards COVID-19.

Materials and Methods: A Cross-sectional study with a sample size of 9000 was conducted for 11 days (Dec 11-21, 2020) including all the 79 wards under Jabalpur Municipal Corporation. Serum samples were tested for the presence of specific antibodies to COVID19 using ICMR-Kavach IgG ELISA kits. The data collected was compiled on Microsoft Excel and data analysis was carried out using STATA 15E statistical software.

Result: Overall seroprevalence of the study population was found to be 28.70% (weighted). Wards of the city of Jabalpur were classified into three categories based on the case prevalence - High (27 wards), Medium (26 wards) and Low (26 wards). Based on the overall seroprevalence, the estimated number of total infections were calculated to be 3, 54,870 for the study population. Overall Case Infection Ratio was 31.41.

Conclusion: The current seroprevalence study rightly provides information on proportion of the population exposed, however, the correlation between presence and absence of antibodies is not a marker of total or partial immunity. Hence, the golden rule of Social distancing, sanitization, personal protective equipment and public health measures have to be continued.

How to cite this article:
Ramaswamy S, Athotra A, Kasar PK, Tiwari R, Tomar SP, Kuraria R et al. Cross-sectional Study on Sero-Prevalence of SARS-CoV-2 Infection in Jabalpur, Madhya Pradesh, India. J Commun Dis 2021; 53(1): 82-88.



Zhu N, Zhang D, Wang W et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl

J Med 2020; 382(8): 727-733.

World Health Organisation. WHO announces COVID-19 outbreak a pandemic. World Health Organisation.

World Health Organization. 2020. p. 1. Available from:


Worldometer. Coronavirus Cases. Worldometer. 2020.pp. 1-22. Available from: https://www.worldometers.


Ministry of Health and Family Welfare. 2020. Available from:

The Lancet. India under COVID-19 Lockdown. Lancet 2021; 395(10233): 1315.

Murhekar M, Bhatnagar T, Selvaraju S et al. SARS-CoV-2 Antibody prevalence in India: findings from the second

Nationwide Household Serosurvey. 2020.

BBC News. India. Feb 5, 2021. ICMR sero survey: One in five Indians exposed to Covid-19. 2021. Available


Padhi A, Kumar S, Gupta E et al. Laboratory Diagnosis of Novel Coronavirus Disease 2019 (COVID-19) Infection.

In: Coronavirus Disease 2019 (COVID-19). Nature Publishing Group 2020. 95-107.

To KKW, Tsang OTY, Leung WS et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples

and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet

Infect Dis 2020; 20(5): 565-574.

Guidance on developing a national deployment and vaccination plan for COVID-19 vaccines. 2020.

Available from:


Jacofsky D, Jacofsky EM, Jacofsky M. Understanding antibody testing for Covid-19. The Journal of

Arthroplasty 2020.

Wikramaratna P, Paton RS, Ghafari M et al. Estimating false-negative detection rate of SARS-CoV-2 by RT-PCR.

medRxiv, 2020.

Li Z, Yi Y, Luo X et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARSCoV-2 infection diagnosis. J Med Virol 2020.

Kumar MS, Bhatnagar T, Manickam P et al. National sero-surveillance to monitor the trend of SARS-CoV-2

infection transmission in India: Protocol for communitybased surveillance. Indian Journal of Medical Research

; 151(5): 419.

Jabalpur District: Census 2011-2020 data. 2021. Available from:


Zimmermann P, Curtis N. Coronavirus infections in children including COVID-19: an overview of the

epidemiology, clinical features, diagnosis, treatment and prevention options in children. The Pediatric

Infectious Disease Journal 2020; 39(5): 355.

Kelvin AA, Halperin S. COVID-19 in children: the link in the transmission chain. The Lancet Infectious Diseases,

Ministry of Rural Development. Poverty measurement in India. Tendulker Committee for BPL. 2021.

Available from:

Shakiba M, Nazari SS, Mehrabian F et al. Seroprevalence of COVID-19 virus infection in Guilan province, Iran.

medRxiv, 2020.

Rosenberg ES, Tesoriero JM, Rosenthal EM et al. Cumulative incidence and diagnosis of SARS-CoV-2

infection in New York. medRxiv, 2020.

Streeck H, Hartmann G, Exner M et al. Vorläufiges Ergebnis und Schlussfolgerungen der COVID-19 Case-

ClusterStudy (Gemeinde Gangelt)[Preliminary results and conclusions from the COVID-19 case-clsuter study

(community Gangelt).

Media Bulletin. Hindustan Times. 2021. Available from:


Media Bulletin. The Hindu. 2020. Available from:


Sakalle S, Saroshe S, Shukla H et al. Seroprevalence of antiSARSCoV2 antibodies in Indore, Madhya Pradesh:

A community-based crosssectional study. J Family Med Prim Care 2020.

Stringhini S, Wisniak A, Piumatti G et al. Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Geneva,

Switzerland (SEROCoV-POP): a population-based study. The Lancet 2020.