Exploring Molecular Diagnostic Approaches for  HPV

Aswini Munirathnam; Nirupa Soundararajan; Mohan kumar; Priyadarshini Shanmugam

Aswini Munirathnam Senior Resident, Department of Microbiology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education (CARE), Kelambakkam, Tamilnadu, India
Nirupa Soundararajan Professor, Chettinad Hospital and Research Institute, Rajiv Gandhi Salai (OMR), Kelambakkam, Tamilnadu, India
Mohan kumar Research scholar, Department of Microbiology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education (CARE), Kelambakkam, Tamilnadu, India
Priyadarshini Shanmugam Professor and Head, Department of Microbiology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education (CARE), Kelambakkam, Tamilnadu, India

Keywords: Human Papillomavirus, Cervical Cancer, Molecular Diagnostics, COBAS HPV Test, HPV DNA Testing, Signal Amplification

Abstract

Introduction: Human Papillomavirus (HPV) is implicated in various anogenital cancers, with developing nations experiencing higher infection rates. Since HPV cannot be cultured in vitro, molecular diagnostic techniques are critical for its detection.
Aim: This study aims to explore and evaluate the various molecular diagnostic approaches available for detecting HPV, emphasising their utility in identifying high-risk HPV strains linked to cervical cancer.
Methods: We review a range of molecular diagnostic techniques, including target amplification methods like Polymerase Chain Reaction (PCR), Reverse Line Blot, Real-Time PCR, and various other advanced HPV DNA testing methods like COBAS HPV Test, Xpert HPV, and Anyplex II HPV 28. Signal amplification techniques such as Digene Hybrid Capture 2 and CARE HPV are also discussed for their efficacy in detecting HPV.
Conclusion: Molecular diagnostic approaches have significantly advanced HPV detection, providing high sensitivity and specificity, particularly for high-risk HPV strains. These techniques are advantageousin the early
diagnosis and management of cervical cancer, particularly in regions with high HPV prevalence. Further refinement and accessibility of these methods are essential for global cervical cancer control.

How to cite this article:
Munirathnam A, Soundararajan N, Kumar A M, Shanmugam P. Exploring Molecular Diagnostic Approaches for HPV. J Commun Dis. 2025;57(3):207-213.

DOI: https://doi.org/10.24321/0019.5138.202587