Evolution Pseudomonas aeruginosa resistance antibiotics isolated from wounds infections and it production Biofilm

Abstract

Abstract

 The current study aimed to detect antibiotics resistance of Pseudomonas aeruginosa and production of the biofilm virulence factor produced by Pseudomonas aeruginosa. (20) Bacterial isolates belonging to the Pseudomonas aeruginosa bacteria were collected from Tikrit Teaching Hospital, and were diagnosed according to microscopic and cultural characteristics and biochemical tests. The results,  appeared resistance 100% to Cephalexin, Clindamycin, Ampicillin, Cefixime, Trimethoprim, Gentamicin,while according to the Concho Red method, showed that 17 (85%) of the bacterial isolates were biofilm producers, with 8 (40%) being strong biofilm producers, and 9 (45%) being weak and moderate biofilm producers, with a percentage of 3 (15%). Not productive. The results, according to the microtiter plate method, showed that the bacterial isolates were biofilm producers: 18 isolates (90%). Where 2 isolates, representing 10%, showed poor productivity, 9 isolates, representing 45%, showed moderate productivity, and 7 isolates, representing 35%, showed strong productivity. The results of the current study showed, through examining the UV-vis spectrophotometer, the maximum absorbance of AuNPs manufactured from... The lion's mane mushroom extract was determined at a wavelength of 540 nanometers, The current results showed that gold nanoparticles manufactured using lion's mane mushroom extract showed good inhibitory activity against the production of biofilms produced by Pseudomonas aeruginosa using three concentrations (50%, 25%, 12.5%), It was found that all isolates that were strong producers of biofilms lost their production capacity after treatment with AuNPs based on the Concho red agar method. However, based on the use of the microtiter plates method, it was found that 4 isolates out of a total of 7 isolates that were strong producers of biofilms, their production decreased to Average production, and 3 isolates changed from strong biofilm producers to weak producers.

key words: anti-biotics , wound infections  , P. aeruginosa