Molecular Detection and Study of some Virulence  Factor Genes Among Pseudomonas aeruginosa  Isolated from Different Human Clinical Specimens

Niran Kadhim F AL-Rubaey; Abdulkadir Kareem Rhumaid Rhumaid; May Yahya Al-Ma’amouri Al-Ma’amouri

Niran Kadhim F AL-Rubaey Assistant Professor, Department of Microbiology, Hammurabi College of Medicine, University of Babylon, Babylon, Babylon, Iraq
Abdulkadir Kareem Rhumaid Rhumaid Department of Technical Medical Laboratories, Institute of Medical Technology Al-Mansour, Middle Technical University
May Yahya Al-Ma’amouri Al-Ma’amouri Department of Technical Medical Laboratories, Institute of Medical Technology Al-Mansour, Middle Technical University

Keywords: Molecular Detection, Pseudomonas aeruginosa, Virulence Genes

Abstract

Introduction: Pseudomonas aeruginosa is an opportunistic pathogen causing various healthcare-associated acute and chronic infections. The multifactorial virulence of the ubiquitous opportunistic pathogen Pseudomonas aeruginosa has been linked to various virulence factors.
Objective: Isolate and identify Pseudomonas auroginosa in different types of human clinical specimens, and evaluate the prevalence of four virulence genes (toxA, exoS, oprL, and oprI) in these isolates.
Methods: The present study examined ninety-seven P. aeruginosa isolates from various types of patient clinical specimens, including wound swabs (n =33), ear discharge samples (n =28), urine samples (n =22) and burn swabs (n =14), during the period from September 2023 to February 2024 in Babylon governorate, Iraq. All colonies of P. aeruginosa were sub-cultured onto the bacterial culture, and then confirmed as P. aeruginosa by using microbiological tests and the API 20E test. Next, the PCR technique was carried out to screen the four
virulence genes (toxA, exoS, oprL, and oprI).
Results: P. aeruginosa was the most frequently isolated organism from wounds, followed by the ear, urine, and burns. The urban community shows a higher prevalence (62.9%) than the rural community (37.1%).
The oprI and oprL genes were the most common genes detected among all tested isolates 97 (100%), while the toxA gene was detected in 79 (81.4%) isolates, and the exoS gene was detected in 68 (70.1%) isolates.
Conversely, the results indicated that oprI, oprL, toxA, and exoS genes were the most commonly found genes in P. aeruginosa isolates from wound swabs, followed by ear discharge samples, urine samples, and
burn swabs.
Conclusion: PCR was empirically found to be the most optimal technique to detect and differentiate the virulence factor genes of Pseudomonas aeruginosa isolates that are involved in human infective diseases.

How to cite this article:
Rajendran R, Baruah K, Anusree S B, Sasi M S. Entomological Surveillance and Risk Assessment for Aedes-borne diseases in the Premises of Medical College Campus Institutions in Thiruvananthapuram Municipal Corporation. J Commun Dis. 2025;57(3):14-24.

DOI: https://doi.org/10.24321/0019.5138.202564

References

Sathe N, Beech P, Croft L, Suphioglu C, Kapat A, Athan E. Pseudomonas aeruginosa: Infections and novel

approaches to treatment “Knowing the enemy” the threat of Pseudomonas aeruginosa and exploring novel

approaches to treatment. Infect Med 2023; 2(3):178194. [PubMed] [Google Scholar]

Li X, Gu N, Huang TY, Zhong F, Peng G. Pseudomonas aeruginosa: A typical biofilm forming pathogen and

an emerging but underestimated pathogen in food processing. Front Microbiol. 2023 Jan 25;13:1114199.

[PubMed] [Google Scholar]