Detection of Insecticide Resistance in Aedes  aegypti from Dengue Endemic Areas of Northern  India

Taruna  Kaura; Seema Devi; Abhishek Mewara; Jaspreet Kaur; Lovleen; NazatInder  Singh; Surya  Kant Sharma; Jaspreet  Takkar; Rakesh  Sehgal; R K  Ratho; Gagandeep  Singh Grover

Taruna Kaura Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Seema Devi Department of Zoology, Lovely Professional University, Jalandhar, Punjab, India.
Abhishek Mewara Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
Jaspreet Kaur NIMR, Community Health Centre, Dhakoli, Punjab, India.
Lovleen Department of Zoology, Lovely Professional University, Jalandhar, Punjab, India.
NazatInder Singh Department of Health and Family Welfare, Chandigarh, India.
Surya Kant Sharma NIMR, Community Health Centre, Dhakoli, Punjab, India.
Jaspreet Takkar Department of Physiology, Gian Sagar Medical College, Punjab, India.
Rakesh Sehgal Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
R K Ratho Department of Virology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Gagandeep Singh Grover Department of Health and Family Welfare, Chandigarh, India.

Keywords: Ae. aegypti, Temephos, Susceptibility, Resistance, Mutation

Abstract

Introduction: Aedes aegypti, a primary vector of arboviral diseases like dengue and chikungunya is distributed widely in the state of Punjab, India. The use of synthetic insecticides and source reduction are the most common methods used to control Aedes populations, although the development of insecticide resistance in Aedes worldwide has become a major challenge. The aim of this study was to investigate the status of resistance of Ae. aegypti to temephos and G119S mutation in the Ace-1 gene which confers resistance toward it.
Method: For this, larval susceptibility to temephos was tested in five districts of Punjab at the WHO recommended concentration of 0.025 mg/L, followed by calculation of LC50 and LC90 at 24 hrs for each district
using log-probit method. Based on larval resistance ratios (RR), the districts were categorised as having mosquitoes that were highly resistant,moderate or susceptible to temephos. The Ace-1 gene was amplified
and sequenced in resistant populations.
Results: We found that Ae. aegypti larvae were resistant to temephos in four out of five districts. Based on LC50 and RR ratios, Ae. aegypti larvae showed moderate resistance in three districts and were highly resistant
in one, and susceptible to temephos in another district. However, the commonly described G119S mutation in the Ace-1 gene was not found in any of the resistant populations.
Conclusion: In conclusion, temephos resistance is developing in Ae. aegypti in the state of Punjab, however, the genetic basis of the same needs further exploration in future studies. There is a need to develop an extensive database of the resistance profile of Ae. aegypti in order to guide the strategic plan of action for the control of Ae. aegypti populations.

How to cite this article:
Kaura T, Devi S, Mewara A, Kaur J, Lovleen, Singh
N, Sharma S K, Takkar J, Sehgal R, Ratho R K,
Grover G S. Detection of Insecticide Resistance
in Aedes aegypti from Dengue Endemic Areas of
Northern India. J Commun Dis. 2024;56(3):56-61.

DOI: https://doi.org/10.24321/0019.5138.202449

References

Weaver SC, Charlier C, Vasilakis N, Lecuit M. Zika, chikungunya, and other emerging vector-borne viral

diseases. Annu Rev Med. 2018;69:395-408. [PubMed] [Google Scholar]

Broeders S, Garlant L, Fraiture MA, Vandermassen E, Suin V, Vanhomwegen J, Dupont-Rouzeyrol M, Rousset D, Van Gucht S, Roosens N. A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses. Int J Infect Dis. 2020;92:160-70. [PubMed] [Google Scholar]

Singh RK, Mittal PK, Kumar G, Dhiman RC. Insecticide susceptibility status of Aedes aegypti and Anopheles

stephensi larvae against temephos in Delhi, India. Int J Mosq Res. 2014;1(3):69-73. [Google Scholar]

Muthusamy R, Shivakumar MS. Susceptibility status of Aedes aegypti (L.) (Diptera: Culicidae) to temephos

from three districts of Tamil Nadu, India. J Vector Borne Dis. 2015;52(2):159-65. [PubMed] [Google Scholar]

Bellinato DF, Viana-Medeiros PF, Araújo SC, Martins AJ, Lima JB, Valle D. Resistance status to the insecticides

temephos, deltamethrin, and diflubenzuron in Brazilian Aedes aegypti populations. Biomed Res Int. 2016;2016:8603263. [PubMed] [Google Scholar]

Garcia GA, David MR, Martins AJ, Maciel-de-Freitas R, Linss JG, Araújo SC, Lima JB, Valle D. The impact

of insecticide applications on the dynamics of resistance: the case of four Aedes aegypti populations

from different Brazilian regions. PLoS Negl Trop Dis. 2018;12(2):e0006227. [PubMed] [Google Scholar]

Grisales N, Poupardin R, Gomez S, Fonseca-Gonzalez I, Ranson H, Lenhart A. Temephos resistance in Aedes

aegypti in Colombia compromises dengue vector control. PLoS Negl Trop Dis. 2013;7(9):e2438. [PubMed]

[Google Scholar]

Misra BR, Gore M. Malathion resistance status and mutations in acetylcholinesterase gene (Ace) in Japanese encephalitis and filariasis vectors from endemic area in India. J Med Entomol. 2015;52(3):442-6. [PubMed] [Google Scholar]

Liebman KA, Pinto J, Valle J, Palomino M, Vizcaino L, Brogdon W, Lenhart A. Novel mutations on the ace1 gene of the malaria vector Anopheles albimanus provide evidence for balancing selection in an area of high insecticide resistance in Peru. Malar J. 2015;14:74. [PubMed] [Google Scholar]

Devi S, Kaura T, Kaur J, Lovleen, Takkar J, Sharma SK, Grover GS. Prevalence of dengue vectors, larval

breeding habitats, Stegomyia indices and their correlation with dengue cases in urban and rural areas of Punjab, India. J Vector Borne Dis. 2020;57(2):176-81. [PubMed] [Google Scholar]

Rueda LM. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission. Zootaxa. 2004;589. [Google Scholar]

Imam H, Zarnigar, Sofi G, Seikh A. The basic rules and methods of mosquito rearing (Aedes aegypti). Trop

Parasitol. 2014;4(1):53-5. [PubMed] [Google Scholar]

World Health Organization. Monitoring and managing insecticide resistance in Aedes mosquito populations:

interim guidance for entomologists. Vol. 16. WHO; 2016. 7 p. [Google Scholar]

Finney DJ. Statistical aspects of monitoring for dangers in drug therapy. Methods Inf Med. 1971;10(1):1-8.

[PubMed] [Google Scholar]

Marcombe S, Poupardin R, Darriet F, Reynaud S, Bonnet J, Strode C, Brengues C, Yebakima A, Ranson H, Corbel V, David JP. Exploring the molecular basis of insecticide resistance in the dengue vector Aedes aegypti: a case study in Martinique Island (French West Indies). BMC Genomics. 2009;10:494. [PubMed] [Google Scholar]

Valle D, Bellinato DF, Viana-Medeiros PF, Lima JB, Martins Junior AJ. Resistance to temephos and deltamethrin in Aedes aegypti from Brazil between 1985 and 2017. Mem Inst Oswaldo Cruz. 2019;114:e180544.

[PubMed] [Google Scholar]

Palomino M, Pinto J, Yañez P, Cornelio A, Dias L, Amorim Q, Martins AJ, Lenhart A, Lima JB. First national-scale

evaluation of temephos resistance in Aedes aegypti in Peru. Parasit Vectors. 2022;15(1):254. [PubMed] [Google Scholar]

Dhiman S, Rabha B, Yadav K, Baruah I, Veer V. Insecticide susceptibility and dengue vector status of

wild Stegomyia albopicta in a strategically important area of Assam, India. Parasit Vectors. 2014;7:295.

[PubMed] [Google Scholar]

Yadav K, Rabha B, Dhiman S, Veer V. Multi-insecticide susceptibility evaluation of dengue vectors Stegomyia

albopicta and St. aegypti in Assam, India. Parasit Vectors. 2015;8:143. [PubMed] [Google Scholar]

Tikar SN, Mendki MJ, Chandel K, Parashar BD, Prakash S. Susceptibility of immature stages of Aedes (Stegomyia) aegypti; vector of dengue and chikungunya to insecticides from India. Parasitol Res. 2008;102(5):907-

[PubMed] [Google Scholar]21. Paeporn P, Komalamisra N, Deesin V, Rongsriyam Y, Eshita Y, Thongrungkiat S. Temephos resistance in two forms of Aedes aegypti and its significance for the resistance mechanism. Southeast Asian J Trop Med Public Health. 2003;34(4):786-92. [PubMed] [Google Scholar]

Melo-Santos MA, Varjal-Melo JJ, Araújo AP, Gomes TC, Paiva MH, Regis LN, Furtado AF, Magalhaes T,

Macoris ML, Andrighetti MT, Ayres CF. Resistance to the organophosphate temephos: mechanisms, evolution

and reversion in an Aedes aegypti laboratory strain from Brazil. Acta Trop. 2010;113(2):180-9. [PubMed][Google Scholar]

Alsheikh AA, Mohammed WS, Noureldin EM, Daffalla OM, Shrwani YA, Hobani KJ, Alsheikh FA, Alzahrani MH,

Binsaeed AA. Studies on Aedes aegypti resistance to some insecticides in the Jazan District, Saudi Arabia. J Egypt Soc Parasitol. 2016;46(1):209-16. [PubMed] [Google Scholar]

Bisset JA, Marín R, Rodríguez MM, Severson DW, Ricardo Y, French L, Díaz M, Pérez O. Insecticide

resistance in two Aedes aegypti (Diptera: Culicidae) strains from Costa Rica. J Med Entomol. 2013;50(2):352-

[PubMed] [Google Scholar]

Bellinato DF, Viana-Medeiros PF, Araújo SC, Martins AJ, Lima JB, Valle D. Resistance status to the insecticides

temephos, deltamethrin, and diflubenzuron in Brazilian Aedes aegypti populations. Biomed Res Int. 2016;2016:8603263. [PubMed] [Google Scholar]

Chediak M, Pimenta Jr FG, Coelho GE, Braga IA, Lima JB, Cavalcante KR, de Sousa LC, de Melo-Santos MA,

Macoris ML, Araújo AP, Ayres CF, Andrighetti MT, Gomes RG, Campos KB, Guedes RN. Spatial and

temporal country-wide survey of temephos resistance in Brazilian populations of Aedes aegypti. Mem Inst

Oswaldo Cruz. 2016;111(5):311-21. [PubMed] [Google Scholar]

Putra RE, Ahmad I, Prasetyo DB, Susanti S, Rahayu R, Hariani N. Detection of insecticide resistance in the

larvae of some Aedes aegypti (Diptera: Culicidae) strains from Java, Indonesia to temephos, malathion and permethrin. Int J Mosq Res. 2016;3(3):23-8. [Google Scholar]

Weill M, Berthomieu A, Berticat C, Lutfalla G, Nègre V, Pasteur N, Philips A, Leonetti JP, Fort P, Raymond M.

Insecticide resistance: a silent base prediction. Curr Biol. 2004;14(14):R552-3. [PubMed] [Google Scholar]

Polson KA, Brogdon WG, Rawlins SC, Chadee DD. Characterization of insecticide resistance in Trinidadian

strains of Aedes aegypti mosquitoes. Acta Trop. 2011;117(1):31-8. [PubMed] [Google Scholar]