Detect the IFNL4 Polymorphism (rs12979860) and level of IFNL4 in serum of Hepatitis C Virus in Iraqi Province

Zahraa Basim Naser; Raya Ezat Maroof; ,Khalied Yassen Zakair

Zahraa Basim Naser College of Health and Medical Techniques, Middle Technical University (MTU), Baghdad, Iraq.
Raya Ezat Maroof College of Health and Medical Techniques, Middle Technical University (MTU), Baghdad, Iraq.
,Khalied Yassen Zakair Kut Technical Institute, Middle Technical University, Baghdad, Iraq.

Keywords: HCV, IFNL4, Detectable Viral Load, Undetectable Viral Load, SNPs

Abstract

Introduction: Hepatitis C virus (HCV) presents a significant and impending threat to the well-being of the global public. In this study, IFNL4 in the serum of HCV patients and SNPs (rs12979860) was to be evaluated in both the patient and control groups.
Method: Sixty specimens were obtained from patients diagnosed with HCV (divided into infected undetectable viral load and detectable viral load), and thirty samples from individuals deemed to be in fair health (control group) from the Teaching Hospital for Gastroenterology and Hepatology, the National Center for Blood Transfusion, and the Al- Wasiti Teaching Hospital. HCV antibodies were detected in all patientsamples, and virus loads were determined. A total of ninety samples were collected from individuals aged 20 to 60 years. The number of viruses was identified by GeneXpert® Systems. ELISA quantified IFNL4. The IFNL4 gene (rs12979860) was determined from blood samples using the polymerase chain reaction and was subsequently transmitted to Macrogen, Korea, for sequencing.
Result: The mean IFNL4 value was significantly higher in the group with undetectable viral load (p < 0.0001). According to the ROC curve study, IFNL4 has 100% sensitivity and 97.0% specificity in the control and undetectable viral load groups. The assay’s specificity is 96.0% for the control group and 97.0% for the detectable viral load group. The primer identifies IFNL4 gene amplification at the rs12979860 region. Geneious software analysis showed SNP variations: CC to CT (rs12979860), GG to GA (rs1288377676), CC to CG (rs1254026524), GG to GT (rs1414880542), GG to GA (rs574801123), and AA to AG (rs1600456091). IFNL4 was more prevalent in the undetectable viral load HCV-infected group.
Conclusion: The presence of genetic polymorphisms in the IFNL4 gene may be of help in planning the treatment course for patients with HCV.

How to cite this article:
Naser B Z, Maroof E R, Zakair Y K . Detect the
IFNL4 Polymorphism (rs12979860) and level
of IFNL4 in serum of Hepatitis C Virus in Iraqi
Province.J Commun Dis. 2024;56(2):22-30.

DOI: https://doi.org/10.24321/0019.5138.202428

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