Expression of Biofilm and Virulence Factors Genes by Commensal and Virulent Staphylococcus epidermidis Isolates
Abstract
Background: The pathogenicity of Staphylococcus epidermidis in community-associated bacterial infections has incredible significance due to its capacity for biofilm production. The goal of this investigation was to compare the expression of different virulence factors among clinical and healthy isolates.
Methods: Six S. epidermidis isolates were confirmed by 16S rRNA, and then phylogenetic tree analysis was used to detect the relationship between clinical and healthy isolates. Quantitative PCR (qPCR) was used to compare the S. epidermidis gene expression level between clinical and healthy isolates. The expression levels of the icaA, clf, and IgG genes were compared in planktonic cells and biofilm form for the same S. epidermidis isolates.
Results: All clinical isolates appeared highly capable of expressing all three genes compared to healthy isolates. The isolate SP137 with robust biofilm formation exhibited significantly higher expression of icaA, clf, and IgG genes (27, 3783.3, and 247.6, respectively) compared to the other isolates (p < 0.01).
Conclusion: Clinical isolates harbor more genes related to virulence factors than healthy individual isolates.It was discovered that all genes (icaA, clf, and IgG) are expressed more strongly in clinical isolates than in healthy persons.
How to cite this article:
Muneam H H, Flayyih M T. Expression of Biofilm
and Virulence Factors Genes by Commensal and
Virulent Staphylococcus epidermidis Isolates. J
Commun Dis. 2024;56(3):66-73.
DOI: https://doi.org/10.24321/0019.5138.202451
Copyright (c) 2024 Journal of Communicable Diseases (E-ISSN: 2581-351X & P-ISSN: 0019-5138)
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