Use of Citrullus colocynthis Callus for Green Synthesis of Silver Nanoparticles and their Activity Against Biofilm-Producing

Marwa Kh. Samawi; Ali A. Alsalihy; Ahmed AbdulJabbar Suleiman

Marwa Kh. Samawi Institute of Genetic Engineering and Biotechnology for Post Graduate Studies , Baghdad University, Baghdad, Iraq
Ali A. Alsalihy Institute of Genetic Engineering and Biotechnology for Post Graduate Studies , Baghdad University, Baghdad, Iraq
Ahmed AbdulJabbar Suleiman Department of Biotechnology, College of Science, University of Anbar, Anbar, Iraq

Keywords: Citrullus colocynthis, Pseudomonas aeruginosa, Silver Nanoparticles, Green Synthesis, Biofilm Formation

Abstract

Introduction: The field of nanotechnology has developed in the recent past and its use has increased in the medical field. This study has attempted to analyse the activity of silver nanoparticles on biofilm
formation in Pseudomonas aeruginosa.
Method: In this study, 30 bacterial samples were used to study the activity of silver nanoparticles on biofilm formation in Pseudomonas aeruginosa. The seeds of Citrullus colocynthis were grown in the laboratory and their leaves were used to produce callus using different hormone concentrations. The callus was grown and was used to produce silver nanoparticles. Ten bacterial isolates (3 strong, 4 medium, and 3 weak) were treated with silver particles to study their effect on biofilm production.
Results: The study showed variation in the ability of bacteria to produce biofilms. It was found that 11 (36.7%) samples had a high ability to form biofilms, 16 (53.3%) had a moderate ability to form biofilms, while the rest of the samples (3, 10%) showed a weak ability to form biofilms.
Conclusion: The study showed a decrease in biofilm production levels for all studied bacterial isolates. This indicated that silver nanoparticles may have the ability to inhibit or reduce biofilm production in Pseudomonas
aeruginosa bacteria.

How to cite this article:
Samawi Kh M, Alsalihy A, Suleiman Ahmed A. Use
of Citrullus colocynthis Callus for Green Synthesis
of Silver Nanoparticles and their Activity Against
Biofilm-Producing. J Commun Dis. 2024;56(2):70-
75.

DOI: https://doi.org/10.24321/0019.5138.202434

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