Detection of Pyocin S and the Effects of Lactobacillus Acidophilus Cell-Free Supernatants on Multi-Drug Resistant Pseudomonas Aeruginosa Isolated from Patients of Baghdad Hospitals

  • Aliaa Ghaffar Husain Bassi Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.
  • Bahaa Abdullah Laftaah Al-Rubaii Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.
Keywords: Pseudomonas aeruginosa, Type S Pyocin, MDR, Resistance Gene, PCR, Lactobacillus acidophilus


Background: The existence of resistance genes in Pseudomonas aeruginosa can be crucial to the pathogenicity of this organism and can cause the bacteria to become resistant to many antibiotic groups. Clinical isolates containing resistance genes must be identified to control the bacteria’s spread and reduce its pathogenicity.
Objectives: To identify the pyocin-producing MDR-Pseudomonas aeruginosa and to examine the expression of several resistance genes in Pseudomonas aeruginosa before and after treatment with a specific concentration of Lactobacillus Acidophilus Cell-Free Supernatants (CFS) by real-time PCR test.
Method: This investigation involved the collection of 350 clinical
specimens from various patients of Baghdad hospitals; 17.1% (60) of all isolates were successfully identified as Pseudomonas aeruginosa. Additionally, the disk diffusion method was used to calculate the minimum inhibitory concentration (MIC) of Pseudomonas aeruginosa. The reverse transcription-PCR technique was then used to find the resistance genes. Finally, gene expression in Lactobacillus acidophilus Cell-Free Supernatants (CFS) was compared before and after treatment using the real-time PCR technique.
Results: Phenotypic testing revealed a high level of antibiotic resistance, whereas genotypic methods revealed the presence of resistance genes and there was a difference in the expression of resistance genes before
and after being treated with CSFs.
Conclusion: Pseudomonas aeruginosa strains exhibit significant levels of pathogenicity, therefore modifications to current antibiotic therapy methods are warranted. Additionally, L. acidophilus CSF showed a positive effect by reducing the expression of certain resistance genes. Consequently, it is possible that in the future, CSF may be used as a substitute treatment for infections caused by Pseudomonas aeruginosa.

How to cite this article:
Bassi A G H, Al-Rubaii B A L. Detection of Pyocin S
in Multi-Drug Resistant Pseudomonas Aeruginosa
Isolated from Patients of Baghdad Hospitals and
the Effects of Cell-Free Supernatants against the
Bacteria. J Commun Dis. 2024;56(1):135-144.



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