Detection of Cytomegalovirus in Patients with Inflammatory Bowel Diseases in Iraq

Hiba  Ahmed Majeed; Haider  Sabah Kadhim; Ali Sameer  Al Shamaa

Hiba Ahmed Majeed Department of Medicine , College of Medicine, Al-Nahrain University, Iraq.
Haider Sabah Kadhim Department of Medicine , College of Medicine, Al-Nahrain University, Iraq.
Ali Sameer Al Shamaa Department of Medicine , College of Medicine, Al-Nahrain University, Iraq.

Keywords: Inflammatory Bowel Disease, Cytomegalovirus, Ulcerative Colitis, Crohn’s Disease

Abstract

Introduction: Inflammatory bowel diseases (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD), are chronic inflammatory disorders of the gastrointestinal tract caused by interactions between genetic,
environmental, immunological, and microbial factors.
Objective: To study the association of Cytomegalovirus in a sample of Iraqi patients with inflammatory bowel diseases
Methodology: The study included 160 patients, of which, 80 were diagnosed with IBD by a specialist in gastroenterology and 80 were apparently healthy subjects. Stool samples were collected from all participants and were prepared for detection of CMV PP65 by ELISA. The molecular method (real-time PCR) was used to detect the DNA of each pathogen.
Results: Twenty-five patients with IBD had a significantly high level (8.44 pg/ml) of CMV as compared to the control group. The results showed that there was no significant correlation between CMV PP65
and the signs and symptoms of IBD patients (smoking, UC severity, bloody diarrhoea, abdominal pain, biological therapy, behaviour, or CD location). CMV real-time copy quantity was significantly correlated
with smoking, bloody diarrhoea, abdominal discomfort, and biological therapy, but not with UC extent, behaviour, or CD location, as determined by molecular real-time PCR.
Conclusion: There is a possible role of CMV in the pathogenesis of IBD in patients in Iraq. The biological treatment given to patients with IBD seems to reduce the CMV level.

How to cite this article:
Majeed HA, Kadhim HS, Shamaa ASA. Detectionof Cytomegalovirus in Patients with Inflammatory Bowel Diseases in Iraq. J Commun Dis. 2023;55(3):67-74.

DOI: https://doi.org/10.24321/0019.5138.202341

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