Entomological Surveillance and Alternate  Methods for Species Identification of Aedes in a  Dengue-endemic District of Punjab, India

Seema Devi; Taruna Kaura; Lovleen; Suryakant Sharma; Adarshpal Kaur; Gagandeep Singh Grover

Seema Devi Lovely Professional University Phagwara, Punjab, India
Taruna Kaura Department of Medical Parasitology, PGIMER, Chandigarh, India.
Lovleen Lovely Professional University Phagwara, Punjab, India.
Suryakant Sharma National Institute of Malaria Research, New Delhi, India
Adarshpal Kaur Department of Health and Family Welfare, Parivar Kalyan, Sec- 34A, Chandigarh, India.
Gagandeep Singh Grover Department of Health and Family Welfare, Parivar Kalyan, Sec- 34A, Chandigarh, India.

Keywords: Surveillance, Identification, Hypopygium, Aedes

Abstract

Background: Entomological surveillance is a vital component of vector control programmes as it helps to determine the geographical distribution, density, and species of vector populations in areas at risk of arboviral diseases. Moreover, calculating Stegomyia indices is an integral component of Aedes surveillance in areas which are at high risk of dengue.

Methodology: In the year 2018, entomological surveillance was carried out in a high dengue endemic district of Punjab to identify the different species of Aedes through morphological identification on the basis of hypopygium. For this, all artificial and natural containers holding stagnant water were inspected for the presence of immature larvae. The larval indices were also recorded.

Results: Aedes breeding was found to be high in urban areas as compared to rural areas. Based on the differences in their morphological characteristics as well as hypopygium structure, three different species of Aedes were identified: Ae. aegypti, Ae. albopictus and Ae. vittatus. Among the three species, the most common species was Ae. aegypti. A positive correlation was recorded between dengue cases and Stegomyia indices.

Conclusion: This is the first study which has recorded the presence of species Ae. vittatus in a district of Punjab, India. The present results indicate that for the identification of damaged specimens, other morphological characteristics must be explored.

How to cite this article:
Devi S, Kaura T, Lovleen, Sharma S, Kaur A, Grover GS. Entomological Surveillance and Alternate
Methods for Species Identification of Aedes in a Dengue-endemic District of Punjab, India. J Commun Dis. 2023;55(2):28-32.

DOI: https://doi.org/10.24321/0019.5138.202321

References

Bhat MA, Krishnamoorthy K. Entomological investigation and distribution of Aedes mosquitoes

in Tirunelveli, Tamil Nadu, India. Int J Curr Microbiol Appl Sci. 2014;3(10):253- 60.

World Health Organization [Internet]. Global strategy for dengue prevention and control 2012–2020; [cited

Apr 3]. Available from: http://apps.who.int/iris/bitstream/handle/10665/75303/9789241504034_eng.

pdf;jsessionid=8BD7C5C13E1CC23A760ADD649728C 11C?sequence=1 [Google Scholar]

Kosoltanapiwat N, Tongshoob J, Singkhaimuk P, Nitatsukprasert C, Davidson SA, Ponlawat A.

Entomological surveillance for Zika and dengue virus in Aedes mosquitoes: implications for vector control in

Thailand. Pathogens. 2020;9(6):442. [PubMed] [Google Scholar]

Balthazar TD, Maia DA, Oliveira AA, Marques WA, Bastos AQ, Vilela ML, Mallet JR. Entomological

surveillance of mosquitoes (Diptera: Culicidae), vectors of arboviruses, in an ecotourism park in Cachoeiras de

Macacu, state of Rio de Janeiro-RJ, Brazil. PLoS One. 2021;16(12):e0261244. [PubMed] [Google Scholar]

Anuradha GN, de Silva WA, Chathuranga WG, Karunaratne SH, Weeraratne TC. Morphological

variations in the male genitalia of seven mosquito genera (Diptera: Culicidae), Sri Lanka. Ceylon J Sci.

;51(Special Issue):509-20. [Google Scholar]

Yadav K, Sarkar PK, Veer V. Utility of male mosquito hypopygium in species identification. Int J Mosq Res.

;1(4):50-4. [Google Scholar]

Sallum MA, Obando RG, Carrejo N, Wilkerson RC. Identification key to the Anopheles mosquitoes

of South America (Diptera: Culicidae). III. Male genitalia. Parasit Vectors. 2020;13:542. [PubMed]

Rueda LM. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue

virus transmission. Zootaxa. 2004;589:1-60. [Google Scholar]

Tyagi BK, Munirathinam A, Venkatesh A. A catalogue of Indian mosquitoes. Int J Mosq Res. 2015;2(2):50-97.

[Google Scholar]

World Health Organization. Guidelines for dengue surveillance and mosquito control. 2nd ed. Regional

Office for the Western Pacific, Manila; 2003. [Google Scholar]

Khalin AV. [Three-dimensionality of the male genitalia shape and species identification in the mosquito genus

Aedes Meigen, 1818 (Diptera, Culicidae)]. Parazitologiia. 2009;43(5):389-410. Russian. [PubMed] [Google

Scholar]

Barraud P J. The fauna of British India, including Ceylon and Burma. Diptera. Vol. 5. Family Culieldae. Tribes

Megarhinini and Culicini. London: Taylor & Francis; 1934. [Google Scholar]

Kaur S, Kirti JS. Relevance of female genitalic attributes in species identification of Culicinae. J Entomol Zool

Stud. 2017;5(6):845-50. [Google Scholar]

Devi S, Kaura T, Kaur J, Lovleen, Takkar J, Sharma S, Grover GS. Prevalence of dengue vectors, larval

breeding habitats, Stegomyia indices and their correlation with dengue cases in urban and rural areas

of Punjab, India. J Vector Borne Dis. 2020;57:176-81. [PubMed] [Google Scholar]

Singh RK, Mittal PK, Kumar G, Karlekar RR, Dhole RB, Dhiman RC. Prevalence of Aedes mosquitoes in various

localities of Gadchiroli district of Maharashtra state, India. Int J Mosq Res. 2015;2(2):38-41. [Google Schola]