Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India

Taruna Kaura; Seema  Devi; Abhishek Mewara; Jaspreet Kaur; Nazat Inder Singh; Lovleen; Surya Kant Sharma; RK Ratho; Rakesh Sehgal; Gagandeep Singh Grover

Taruna Kaura Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research Sector 12, Chandigarh, India.
Seema Devi Lovely Professional University, Jalandhar, Delhi, G.T. Road, Phagwara, Punjab, India.
Abhishek Mewara Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research Sector 12, Chandigarh, India.
Jaspreet Kaur Department of Health and Family Welfare, Parivar Kalyan Bhawan, Sector-34 A, Chandigarh, India.
Nazat Inder Singh NIMR, Field Site, First Floor, Community Health Centre Dhakoli, S.A.S. Nagar, Punjab, India.
Lovleen Lovely Professional University, Jalandhar, Delhi, G.T. Road, Phagwara, Punjab, India.
Surya Kant Sharma Department of Health and Family Welfare, Parivar Kalyan Bhawan, Sector-34 A, Chandigarh, India.
RK Ratho Department of Virology, Postgraduate, Institute of Medical Education and Research, Sec-12, Chandigarh, India.
Rakesh Sehgal Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research Sector 12, Chandigarh, India.
Gagandeep Singh Grover NIMR, Field Site, First Floor, Community Health Centre Dhakoli, S.A.S. Nagar, Punjab, India.

Keywords: Susceptible, Resistance, VGSC, Mutation, Adulticides, Polymorphism

Abstract

Background: As the state of Punjab has become endemic for dengue, this study was planned to determine the susceptibility status for two different classes of adulticides and the VGSC gene polymorphism in domain II of Aedes aegypti.
Methodology: Adult bioassays were performed with pyrethrum, deltamethrin and malathion as per the WHO protocol. AS-PCR and sequencing of VGSC gene were carried out to detect V1016G and Kdr (Knockdown resistance) mutations.
Results: Ae. aegypti from the districts of Ludhiana and Patiala were found to be resistant to pyrethrum. The V/ V genotype frequency was found to be higher in all districts. Three known polymorphisms in VGSC gene were not recorded but one synonymous and non-synonymous mutation was found. Group B intron was found in domain II of the VGSC gene. For deltamethrin and malathion, 100% susceptibility was recorded for all four districts.
Conclusion: Thus, the present findings indicate 100% susceptibility towards deltamethrin and malathion whereas, moderate resistance towards pyrethrum was recorded. Therefore, more studies should be planned to study polymorphisms in the various domains of the VGSC gene.

How to cite this article:
Kaura T, Devi S, Mewara A, Kaur J, Singh NI, Lovleen, Sharma SK, Ratho RK, Sehgal R, Grover GS. Detection of Insecticide Susceptibility Status and KDR Mutation in Field-Collected Aedes Aegypti from Different Districts of Punjab, India. J Commun Dis. 2022;54(4):21-28.

DOI: https://doi.org/10.24321/0019.5138.202298

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