Antimalarial Activity of the Crude Extract and Solvent Fractions of the Stem of Momordica Charantia in Plasmodium Berghei Infected Mice

Antimalarial activity of Momordica charantia

  • Akintola AO Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
  • Kehinde BD Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
  • Ayoola PB Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
  • Ibikunle GJ Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
  • Oyewande EA Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
  • Arotayo RA Department of Pure and Applied Chemistry, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
  • Akwu Bala Peter Department of Anatomy, Faculty of Basic Medical Sciences, Kogi State University, Anyigba, Nigeria.
  • Bello MO Department of Pure and Applied Chemistry, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Keywords: Momordica charantia, Antimalarial, Solvent fractions, Plasmodium berghei, Crude Extract, Antiplasmodial


Introduction: The emergence and rapid spread of multidrug-resistant Plasmodium strains, especially Plasmodium falciparum, has become a major concern for health professionals when it comes to malaria
prophylaxis and treatment, limiting medication options, necessitating the search for new antimalarial drugs derived from plants. In mice infected with Plasmodium berghei, the antimalarial function of Momordica
charantia stem crude methanolic extract and solvent fractions (hexane, ethyl acetate, and aqueous) was examined.

Method: Starting on the day the infection was identified, the extract and fractions were administered continuously for four days. Tween 80 (0.3 ml) was given to the control group, while the standard reference
drugs were chloroquine (10 mg/kgbw) and arteether (3 mg/kgbw) which were given for three days. The crude extract and fractions were tested for antimalarial activity in Plasmodium berghei infected mice using a
four-day suppressive test.

Result: At 500 mg/kgbw, the crude extract, hexane fraction, ethyl acetate fraction, and aqueous fraction developed 80.62, 90.09, 91.23, and 81.72 per cent chemosuppression respectively, on day 6 after infection.
Chemosuppression was 100% for chloroquine and 90% for arteether.

Conclusion: These results showed that the crude extract and solvent fractions of Momordica charantia stem had antiplasmodial efficacy comparable to the reference drug, indicating that the plant could be used as a natural antimalarial agent.

How to cite this article:
Antimalarial Activity of the Crude Extract and Solvent Fraction of the Stem of Momordica Charantia in Plasmodium Berghei Infected Mice. J Commun Dis. 2022;54(3):37-47.



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