Burden of Rotavirus (RV) Infection in Children Below Five Years of Age Having Acute Diarrhoea and Pattern of Circulating Genotypes in a Community in Indore City of Madhya Pradesh
Background: Determining the prevalence of rotavirus in a specific
area can provide valuable insights regarding its seasonality, aid the
healthcare system in effectively managing cases of acute diarrhoea,
and contribute to the prevention of drug misuse.
Aim: To study the burden of rotavirus infection in children below five
years of age having acute diarrhoea and pattern of circulating genotypes
in a community in Indore city of Madhya Pradesh.
Methods: Children below five years of age with acute diarrhoea provided
a total of 100 stool samples, which were then tested for the presence
of rotavirus antigen using an enzyme immunoassay (EIA). After that,
RT-PCR was used to genotype the EIA-positive samples’ G and P types.
Demographic and clinical information were recorded on a proforma.
Results: The prevalence of rotavirus infection in children below five
years of age having acute diarrhoea was 17%. Nine typeable rotavirus
strains out of a total of 17 were typed using RT-PCR. One strain (11.1%)
was partially typed, two strains (22.2%) were not typeable, and six
strains (66.7%) were fully genotyped for the G and P genotypes. The
G1 genotype was found in the greatest number of strains (5, 71.4%),
followed by G2 and G10 in 1 strain each (14.3% each). P8 and P4 were
found in one strain each (50.0% each).
Conclusion: The majority of rotavirus strains were G1 genotyped. In
order to boost the regional specificity of multivalent RV vaccines, this
study emphasises the need to include new rotavirus strains.
How to cite this article:
Gupta A, Shah H. Burden of Rotavirus (RV)
Infection in Children Below Five Years of Age
Having Acute Diarrhoea and Pattern of Circulating
Genotypes in a Community in Indore City of
Madhya Pradesh. Chettinad Health City Med J.
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