In-silico and In-vitro Evaluation of the Anti-diabetic Potential of pâ€‘Propoxybenzoic Acid
Background: p-propoxybenzoic acid (p-PBA) is reported as an active chemical constituent of medicinal plants that possess anti-diabetic activity. It is termed a Multiple-Designed Ligand (MDL) having the ability to block more than one enzyme. A molecular docking study justifies the binding ability of pâ€‘PBA with acarbose and NaVO4 which were considered standard compounds having the ability to block target enzymes. Î±-amylase inhibition assay was used as an in-vitro screening model to evaluate the activity of p-PBA against diabetes on an initial basis.
Methods: For the molecular docking study, a PDB file of p-PBA was prepared and PDB files of Î±â€‘amylase (1C8Q), Î±-glucosidase (5KZW) and PTP1B (5K9W) were procured. p-PBA was docked against the enzymes using the blind docking method. The binding score of p-PBA and standard with enzymes was obtained and compared. The percentage inhibition of an Î±-amylase enzyme by p-PBA was measured by using a DNSâ€‘modified Î±-amylase inhibition assay and half-maximal inhibitory concentration (IC50) was calculated.
Results: p-PBA has a significant inhibitory effect against Î±-amylase, Î±-glucosidase, and PTP1B with docking scores of 8.43 Â± 0.44 kcal/mol, 9.19 Â± 0.49 kcal/mol, and 9.40 Â± 0.47 kcal/mol respectively. IC50 calculated from the results of Î±-amylase inhibition assay p-PBA was 56.59 Î¼g/mL.
Conclusion: A combination of in-silico and in-vitro methods assessed p-PBAâ€™s anti-diabetic potential on an initial basis. A molecular docking study involving p-PBA concluded the affinity of p-PBA to Î±â€‘amylase, Î±-glucosidase, and PTP1B was significantly correlated with the affinity of acarbose and NaVO4. In-vitro Î±â€‘amylase assay validated the compoundâ€™s inhibitory action against the enzyme.
How to cite this article:
Raval K, Tirgar P. In-silico and In-vitro Evaluation of the Anti-diabetic Potential of pâ€‘Propoxybenzoic Acid. Chettinad Health City Med J. 2023;12(1):39-44.
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