APCRI Journal

Virus Hybrid Vectored Particles Carrying an RNA Coding for Rabies Virus Glycoprotein

Thaissa Consoni Bernardino — 2023-12-30

Introduction: This study was aimed at establishing a system for producing virus hybrid vectored particles (VHVP) capable of carrying the RNA of the rabies virus glycoprotein (VHVP-RVGP).
Method: To produce VHVP-RVGP, HEK 293T cells were co-transfected with pCMV-Gag-Pol (from leukemia murine virus), pCMV-E1E2 (glycoproteins of hepatitis C virus), and pCMV-RVGP. VHVP-RVGP were collected 48 hours post-co-transfection and titrated by quantitative RT-PCR (RTq-PCR). The RVGP expression was detected by ELISA in lysates of Huh-7 cells treated with VHVP-RVGP.
Results: RT-qPCR assays that were conducted for the detection of cell attachment, as well as entry of VHVP-RVGP, revealed that only a small part was able to enter Huh-7 cells, indicating a limitation in the transduction process. Western blotting assays showed that MLV’s Gag-Pol protein production as well as the incorporation of HCV E1E2 into VHVP were successful.
Conclusion: In conclusion, the data demonstrated that the VHVP system was efficient in carrying the RNA-RVGP, although the HCV E1 and E2 incorporation was not efficiently presented for a proper VHVP conformation.

How to cite this article:
Bernardino TC, Suarez SFP, Pereira CA, Astray
RM, Coroadinha AS, Soares H, Jorge SAC. Virus
Hybrid Vectored Particles Carrying an RNA
Coding for Rabies Virus Glycoprotein. APCRI J.
2023;25(2):1-7.

DOI: https://doi.org/10.24321/0973.5038.202308

Allergic Manifestations to Equine Rabies Immunoglobulin: A Case Series

Saramma Abraham — 2023-12-30

Introduction: Rabies is a vaccine preventable zoonotic disease, however, if not prevented in time, it may become 100% fatal. Combining vaccine- immunoglobulin therapy with proper wound care is advised for effective rabies prevention. Equine rabies immunoglobulin is cheaper than human rabies immunoglobulin, but there is an increased chance of hypersensitivity reaction following its administration.
Method: A case series study has been presented in this article consisting of three different presentations of hypersensitivity reactions after the administration of equine rabies immunoglobulin.
Results: Two cases developed an immediate allergic reaction after desensitization. The third case showed a delayed local reaction and did not develop any allergy during the skin test before administering the full dose.
Conclusion: In a poor resource setting like India, skin sensitivity testing may be continued to identify high risk patients, and provide desentization, but still allergic reactions may be anticipated for them.

Unavailability of Dedicated Dosage Vials of Anti-rabies Vaccine and Immunoglobulin for Intradermal Route Despite its Proven Advantage over Intramuscular Route

Saurabh Pandey — 2023-12-30

How to cite this article:
Pandey S, Bharti O, Reddy DH. Unavailability of
Dedicated Dosage Vials of Anti-rabies Vaccine and
Immunoglobulin for Intradermal Route Despite
its Proven Advantage over Intramuscular Route.
APCRI J. 2023; 25(2): 11-12.

DOI: https://doi.org/10.24321/0973.5038.202309