Estimation of the Antibiotic Activity against Pseudomonas spp Isolated from Ear Infection
Abstract
Background: An ear infection, can be classified into otitis externs and otitis media, this affects all age groups especially infants and young. This infection associate with pathogenic microorganism type, frequent antibiotic uses, health care and age. The most common pathogenic bacteria of the ear infection are Pseudomonas spp. Antibiotic resistance represents a serious threat to the health of humans.
Methods: 48 ear swabs were collected through the use of wooden sticks in a sterile container for the identification of Pseudomonas, Serratia and Klebsiella species by VITEK 2 system and biochemical tests. The antibiotic susceptibility against these bacterial species was detected through the method of standard disk diffusion on the Moller Hinton agar.
Results: Among ear swabs, the positive growth percentage of the pathogenic gram-negative bacteria was 29.166%, and the percentage of Pseudomonas spp. was 57.142%. Males were found to be more susceptible than females with an infection percentage of 57.142%.
Conclusion: The antibiotic susceptibility patterns show the azithromycin, gentamycin, piperacillin, ceftriaxone, cefepime, imipenem, ceftriazon, and gentamycin have activity against Pseudomonas spp.
How to cite this article:
Hussein EF. Estimation of the Antibiotic Activity against Pseudomonas spp Isolated from Ear Infection. J Commun Dis. 2021;53(3):227-231.
DOI: https://doi.org/10.24321/0019.5138.202161
References
Gorems K, Beyene G, Berhane M, Mekonnen Z. Antimicrobial susceptibility patterns of bacteria isolated
from patients with ear discharge in Jimma Town, Southwest, Ethiopia. BMC Ear Nose Throat Disord.
;18:17. [PubMed] [Google Scholar]
Adoga AS, Ma’an EN, Malu D, Badung BP, Obiesie IV, Nwaorgu OG. Swab and aspiration specimen collection
methods and antibiogram in chronic suppurative otitis media at Jos University Teaching Hospital: which is
superior? Ann Afr Med. 2010;9(4):230-4. [PubMed] [Google Scholar]
Mesfin W, Muluken B. Bacterial isolate and antibacterial resistance pattern of ear infection among patients
attending at Hawassa university referral Hospital, Hawassa, Ethiopia. Ind J Otol. 2014;20(4):155-8.
[Google Scholar]
Wasihun AG, Zemene Y. Bacterial profile and antimicrobial susceptibility patterns of otitis media
in Ayder Teaching and Referral Hospital, Mekelle University, Northern Ethiopia. Springerplus. 2015;4:701.
[PubMed] [Google Scholar]
Woodfield G, Dugdale A. Evidence behind the WHO guidelines: hospital care for children: what is the most
effective antibiotic regime for chronic suppurative otitis media in children? J Trop Pediatr. 2008;54(3):151-6.
[PubMed] [Google Scholar]
Chirwa M, Mulwafu W, Aswani JM, Masinde PW, Mkakosya R, Soko D. Microbiology of chronic
suppurative otitis media at Queen Elizabeth Central Hospital, Blantyre, Malawi: a cross-sectional descriptive
study. Malawi Med J. 2015;27(4):120-4. [PubMed] [Google Scholar]
Bluestone CD, Klein JO. Otitis Media in Infants and Children. 4th ed. Hamilton, Ontario, Canada: BC Decker,
Inc; 2007. [Google Scholar]
Ulug M, Oksay B. Bacterial isolates and antibiotic susceptibility patterns of ear discharge from patients
with chronic suppurative otitis media and review of the literature. Sylwan. 2014;158(6):1-25. [Google Scholar]
Afolabi OA, Salaudeen AG, Ologe FE, Nwabuisi C, Nwawolo CC. Pattern of bacterial isolates in the middle
ear discharge of patients with chronic suppurative otitis media in a tertiary hospital in north central Nigeria.
Afr Health Sci. 2012;12(3):362-7. [PubMed] [Google Scholar]
Al Hazzaa SAF, Tabbara KF, Gammon JA. Pink hypopyon: a sign of Serratia marcescens endophthalmitis. Br J
Ophthalmol. 1992;76(12):764-5. [PubMed] [Google Scholar]
Abera B, Kibret M. Bacteriology and antimicrobial susceptibility of otitis media at dessie regional health
research laboratory, Ethiopia. Ethiop J Health Dev. 2011;25:161‑7. [Google Scholar]
Edwin B, Prasanna V, Kannan I, Katiyar VH, Dhanapal E. Incidence of bacterial colonization in the oropharynx
of patients with ear, nose and throat infections. Int J Med Sci Public Health. 2014;3(8):931-4.
World Health Organization [Internet]. Antimicrobial resistance: Global Report on Surveillance. 2014.
Available from: https://reliefweb.int/report/world/antimicrobial-resistance-global-report-surveillance-
?gclid=CjwKCAjwkvWKBhB4EiwA-GHjFslODFiofjWOqZvFITHsSN5lunXsz9RbJcW8YQMpFRjfbAt4CETfBoCqxgQAvD_BwEAddas F, Algethami M,
Mahmalji N, Zakai S, Alkhatib T. Bacterial etiology and antimicrobial sensitivity patterns of ear infections
at King Abdulaziz University Hospital, Jeddah, Saudi Arabia. J Nat Sci Med. 2019;2:147-52. [Google Scholar]
Farhan EA, Pooja KK, Najla AA, Anum DA, Geeta B. Ear infections in Karachi: the frequency and antibiotic
resistance of bacterial isolates. Pak J Med Sci. 2011;27(1):177-81. [Google Scholar]
Ahmad S. Antibiotics in chronic suppurative otitis media: a bacteriologic study. Egypt J Ear Nose Throat
Allied Sci. 2013;14:191-4. [Google Scholar]
Muluye D, Wondimeneh Y, Ferede G, Moges F, Nega T. Bacterial isolates and drug susceptibility patterns
of ear discharge from patients with ear infection at Gondar University Hospital, Northwest Ethiopia. BMC
Ear Nose Throat Disord. 2013;13(1):10. [PubMed] [Google Scholar]
Seid A, Deribe F, Ali K, Kibru G. Bacterial otitis media in all age group of patients seen at Dessie referral
hospital, North East Ethiopia. Egypt J Ear Nose Throat Allied Sci. 2013;14:73-8. [Google Scholar]
Hailu D, Mekonnen D, Derbie A, Mulu W, Abera B. Pathogenic bacteria profile and antimicrobial
susceptibility patterns of ear infection at Bahir Dar Regional Health Research Laboratory Center, Ethiopia.
Springerplus. 2016;5:466. [PubMed] [Google Scholar]
Argaw-Denboba A, Abejew AA, Mekonnen AG. Antibiotic-resistant bacteria are major threats of otitis
media in Wollo Area, Northeastern Ethiopia: a tenyear retrospective analysis. Int J Microbiol. 2016:1-6.
[PubMed] [Google Scholar]
Mittal R, Lisi CV, Gerring R, Mittal J, Mathee K, Narasimhan G, Azad RK, Yao Q, Grati M, Yan D, Eshraghi
AA, Angeli SI, Telischi FF, Liu XZ. Current concepts in the pathogenesis and treatment of chronic suppurative
otitis media. J Med Microbiol. 2015;64(10):1103-16. [PubMed] [Google Scholar]
Anie CO, Enwa FO, Iyamu MI, Asoro PE. Incidence and antibiotics susceptibility pattern of bacteria of
ear origin. IJPSR. 2020;41:3427-32. [Google Scholar]
Kittinger C, Lipp M, Baumert R, Folli B, Koraimann G, Toplitsch D, Liebmann A, Grisold AJ, Farnleitner AH,
Kirschner A, Zarfel G. Antibiotic resistance patterns of Pseudomonas spp. isolated from the river Danube.
Front Microbiol. 2016;7:586. [PubMed] [Google Scholar]
Uddén F, Filipe M, Reimer Å, Paul M, Matuschek E, Thegerström J, Hammerschmidt S, Pelkonen T, Riesbeck
K. Aerobic bacteria associated with chronic suppurative otitis media in Angola. Infect Dis Poverty. 2018;7(1):42.
[PubMed] [Google Scholar]
Appiah-Korang L, Asare-Gyasi S, Yawson AE, Searyoh K. Aetiological agents of ear discharge: a two year
review in a teaching hospital in Ghana. Ghana Med J. 2014;48(2):91-5. [PubMed] [Google Scholar]
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