Methods of detecting Helicobacter pylori bacteria in patients with stomach ulcers and examining its resistance to antibiotics

Abdullah  Zuhair; Abdul                 Karim; Maali Alhayali; Wael Thanoon Younis Alzubaidi

Abdullah Zuhair Instructor,college of Medicine,University of Mosul,Mosul,Iraq.
Abdul Karim Instructor,college of Medicine,University of Mosul,Mosul,Iraq.
Maali Alhayali Alhadba Specialized Hospital, Mosul, Iraq
Wael Thanoon Younis Alzubaidi College of Medicine, University of Mosul, Mosul, Iraq

Keywords: Helicobacter pylori ,Rapid Urease test , culture, Antibiotic,PCR.

Abstract

Background: One intestinal organism that has infected over half of the world's population is Helicobacter pylori. The aim of this study was to isolate and diagnose Helicobacter pylori and determine its resistance to antibiotics from patient samples by different methods.

Methods: Participants in this study included 125 patients with stomach issues of various ages, both sexes, and genders (60 men and 65 women). They underwent diagnostic upper gastrointestinal endoscopy at the "Endoscopy Unit of Ibn Sina Hospital and Private Clinic in Mosul" during the period from November 2023 to January 2024.

Results: The results showed significant differences between patients diagnosed by endoscopy and rapid urease test, where 76 patients out of 105 infected patients had positive results. We obtained 40 positive isolates from the culture test, and antibiotic resistance was tested for Helicobacter pylori isolates, which showed resistance to amoxicillin and metronidazole, while sensitivity to clarithromycin and tetracycline appeared in about 90% of the diagnosed cases.

Conclusion: The findings demonstrated that following genetic confirmation by 16SrRNA, the sensitivity of culture for identifying H. pylori was more dependable than that of other tests. With a rate of 72.38%, the urease cassette test produced positive results in less than 30 minutes. This test is a quick and inexpensive way to diagnose H. pylori.

How to cite this article:
Karim A Z A, Alhayali M, Alzubaidi W T Y. Evaluating Different Methods of Helicobacter pylori Detection and Antibiotics Resistance. J Commun Dis. 2025;57(2):82-87.

DOI: https://doi.org/10.24321/0019.5138.202541

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