Larvicidal Activity of Phenylbutanoid Isolated from the Rhizome of Zingiber montanum (J. Koenig) against Aedes albopictus (Skuse) and Cx. quinquefasciatus Say
Abstract
Introduction: Mosquito-borne infections are most prevalent in the northern districts of West Bengal, India. The public health administrator in this region relies heavily on synthetic insecticides, which has created
more challenges than solving problems. This study attempts to find ecofriendly, biodegradable, and effective botanicals against mosquitoes, especially Aedes albopictus and Culex quinquefasciatus.
Methods: A preliminary screening study showed that the n-hexane extract of Zingiber montanum rhizome possessed potential larvicidal activity against mosquito larvae. Bioassay-guided fractionation led to
the isolation of bioactive phenylbutanoid which was characterised using 1H and 13C NMR and HRMS-TOF.
Results: The compound was identified as cis-1,2-Bis-[(E)-3,4- dimethoxystyryl]cyclobutane (DMSCB) by comparing with previously published data. The molecular ion of the compound was found to be 381.261 m/z with a molecular formula of C24H28O4. The compound was found to have LC50 values of 39.951 ppm and 58.178 ppm against Ae. albopictus and Cx. quinquefasciatus larvae respectively.
Conclusions: Phenyl propanoids and terpenoids are known to possess some level of insecticidal activity but our studies show that phenylbutanoid, DMSCB, also possesses insecticidal activity. This implies that plants containing phenylbutanoid affect the overall insecticidal activity of an extract. Therefore, the outcome of our study suggests that the rhizome extract of Z. montanum has the potential to be used in field studies.
How to cite this article:
Subba A, Saha D. Larvicidal Activity of Phenylbutanoid Isolated from the Rhizome of Zingiber montanum (J. Koenig) against Aedes albopictus (Skuse) and Cx. quinquefasciatus Say. J Commun Dis. 2023;55(4):63-70.
DOI: https://doi.org/10.24321/0019.5138.202357
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