Characterisation of Antibiotic Genes of Acinetobacter baumannii Isolated from Patients in Baghdad Hospitals
Abstract
Introduction: The gram-negative bacterium known as Acinetobacter baumannii is frequently found in soil and water, as well as in samples taken from animals and humans. This study isolated and identified
50 urine samples from four Baghdad educational hospitals. Method: Biochemical testing, selective synthetic media (CHROMagar), and polymerase chain reaction identified all bacterial samples. Only 20
isolates were identified as A. baumannii using a primer targeting the blaOxa-51 gene. Antibiotic susceptibility was tested using disc diffusion with eight antibiotics.
Results and Conclusion: The susceptibility testing of A. baumannii to antibiotics revealed that this bacterium had a higher resistance towards cefotaxime (8, 40%), followed by imipenem (6, 30%), gentamycin (5,
15%), and trimethoprim (2, 10%). The resistance was the same for both tetracycline and tobramycin (3, 15%). In contrast, the antibiotics ampicillin-sulbactam and doxycycline encountered a lower level of resistance, specifically 1% and 5% respectively. Analysis of 20 DNA samples of A. baumannii revealed the presence of OmpA and CsuE genes, with a gene size of 168 bp and 162 bp, respectively. The percentage of these genes in the samples was found to be 60%.
How to cite this article:
Hasan O F, Najm B Y, Majeed H M, Khallel S H. Characterisation of Antibiotic Genes of Acinetobacter baumannii Isolated from Patients in Baghdad Hospitals. J Commun Dis. 2023;55(4):38-45.
DOI: https://doi.org/10.24321/0019.5138.202352
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