Fractional Extraction, Isolation and Identification of Biologically Active Compounds from Anamirta cocculus (L.) Wight & Arn to Control Culex quinquefasciatus Say and Aedes albopictus Skuse
Background & Objectives: Development of resistance towards synthetic insecticides is one of the major problems in vector control programmes and it can be reduced to an extent by the alternate use of botanical or bio insecticides. The present study aims to find out the larvicidal activities of Anamirta cocculus (L.) Wight and Arn against Culex quinqueafsciatus Say and Aedes albopictus Skuse and to isolate the most active compounds present in the active fraction of the plant extract.
Methods: Cold extracts of A. cocculus seeds were taken using methanol as solvent and fractionation was done using the solvents n-hexane, ethyl acetate and acetone by column chromatographic method. Bioassays were conducted using all these extracts and LC50 were calculated using the probit analysis developed by Finney. The structural elucidation of the compounds of most active fraction isolated from chromatographic studies after bioassay was done by Liquid chromatography–mass spectrometry (LCMS) and Nuclear magnetic resonance (NMR) spectroscopy.
Result: A. cocculus treated larvae exhibited more restlessness, convulsions and sluggishness, and the restless activity is more in Ae. albopictus than in Cx. quinquefasciatus. The activity of column fractions of A. cocculus was in the order Hexane (H) > Acetone (A) > H:EA (3:1) >H:EA (2:1)> H:EA (1:1): Ethyl acetate (EA): H:EA (1:2) >H:EA (1:3). The compounds present in the most active fractions were Picrotin and Menispermine respectively.
Interpretation & Conclusion: From the results it has been proved that the plant is having potential larvicidal activity.
How to cite this article:
Anju Viswan K, Pushapalatha E, Arivudainambi S, Selvamuthukumaran T. Fractional Extraction, Isolation and Identification of Biologically Active Compounds from Anamirta cocculus (L.) Wight & Arn to Control Culex quinquefasciatus Say and Aedes albopictus Skuse. J Commun Dis 2020; 52(3): 53-57.
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